The role of intestinal dendritic cells subsets in the establishment of food allergy.

Smit JJ, Bol-Schoenmakers M, Hassing I, Fiechter D, Boon L, Bleumink R, Pieters RH

The role of intestinal dendritic cells subsets in the establishment of food allergy.

Clin Exp Allergy 2011 Apr 8.

Background Food allergy affects approximately 6% of children and is the leading cause of hospitalization for anaphylactic reactions in westernized countries. Crucial in the establishment of allergy is the activation of dendritic cells (DC) leading to T helper 2-mediated responses. Objective We, therefore, investigated whether changes in DC subsets precede the establishment of food allergy, and which DC subsets have functional relevance during allergic sensitization in a mouse model. Methods Changes in DC populations in the intestine were analysed after exposure to cholera toxin alone and in combination with peanut extract (PE) as an allergen. To study the functional role of DC subsets in relation to food allergy, we used expansion of DC in vivo by treatment with Flt3L.

Results Sensitization to PE in this mouse model was accompanied by a shift in DC subsets in intestinal tissues towards more CD11b(+) DC and less CD103(+) DC. No significant changes in the plasmacytoid DC (pDC) numbers were observed. Flt3L treatment, resulting in the expansion of all DC subtypes, inhibited allergic manifestations in our model, including Th2 cytokine production, PE-specific IgE and PE-induced mast cell degranulation. pDC depletion reversed Flt3L-induced inhibition of IgE responses and mast cell degranulation.

Conclusions and Clinical Relevance The establishment of food allergy is accompanied by profound changes in DC subsets in the intestine towards more inflammatory CD11b(+) DC. In addition, expansion of DC numbers by Flt3L, in particular pDC, inhibits the establishment of allergic manifestations in the intestine. These findings are of relevance for understanding the role of DC subsets early during the process of allergic sensitization, and may lead to new therapeutic or prophylactic opportunities to prevent food allergy

Source : http://www.unboundmedicine.com/

Provided by

CHILDREN ALLERGY CLINIC ONLINE
JL Taman bendungan Asahan  5 Jakarta Pusat, Jakarta Indonesia 10210

Phone : (021) 70081995 – 5703646
htpp://www.allergyclinic.wordpress.com/

Clinical and Editor in Chief :
Dr Widodo judarwanto SpA
email : judarwanto@gmail.com,

Information on this web site is provided for informational purposes    only and is not a substitute for professional medical advice. You  should   not use the information on this web site for diagnosing or  treating a   medical or health condition. You should carefully read all  product   packaging. If you have or suspect you have a medical problem,  promptly   contact your professional healthcare provider.

Copyright © 2011, Allergy Clinic Online Information Education Network. All rights reserved

Tinggalkan Balasan

Isikan data di bawah atau klik salah satu ikon untuk log in:

Logo WordPress.com

You are commenting using your WordPress.com account. Logout / Ubah )

Gambar Twitter

You are commenting using your Twitter account. Logout / Ubah )

Foto Facebook

You are commenting using your Facebook account. Logout / Ubah )

Foto Google+

You are commenting using your Google+ account. Logout / Ubah )

Connecting to %s